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KMID : 0359319920320030389
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Korean Journal of Veterinary Research
1992 Volume.32 No. 3 p.389 ~ p.398
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Construction of recombinant DNA clone for bovine viral diarrhea virus
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Cho, H . J .
Masri, S . A ./여상건./Yeo, Sang Geon
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Abstract
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Molecular cloning was carried out on the Danisli strain of bovine viral diarrhea virus(BVDV) to construct stcategy for the diagnostic tools and effective vaccine of BVD afterwards. A recombinant DNA clone(No. 29) was established successfully from cDNA for viral RNA tailed with adenine homopolymer at 3´-end.
^(32)P-labeled DNA probes of 300∼1, 800bp fragments, originating from the clone 29, directed specific DNA-RNA hybridization results with BVDV RNA. Recombinant DNA of the clone 29 was about 5,200bp representing 41.6% of the full length of Danish strain´s RNA, and restriction sites were recognized for EcoR I, Sst I, Hin III and Pst I restriction enzymes in the DNA fragment.
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